chemi doc eq densitometer Search Results


chem doc densitometry software  (Bio-Rad)
93
Bio-Rad chem doc densitometry software
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Chem Doc Densitometry Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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densitometer  (Bio-Rad)
99
Bio-Rad densitometer
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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densitometer - by Bioz Stars, 2026-05
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computer densitometer  (Bio-Rad)
96
Bio-Rad computer densitometer
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Computer Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computer densitometer - by Bioz Stars, 2026-05
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chemi doc system  (Bio-Rad)
99
Bio-Rad chemi doc system
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Chemi Doc System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad image software  (Bio-Rad)
96
Bio-Rad bio rad image software
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Bio Rad Image Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemi doc image lab 5 1  (Bio-Rad)
99
Bio-Rad chemi doc image lab 5 1
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Chemi Doc Image Lab 5 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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computing densitometer  (Bio-Rad)
96
Bio-Rad computing densitometer
Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc <t>densitometry</t> software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.
Computing Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc densitometry software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.

Journal:

Article Title: Enterolactone Inhibits Insulin-Like Growth Factor-1 Receptor Signaling in Human Prostatic Carcinoma PC-3 Cells 1 2

doi: 10.3945/jn.108.101832

Figure Lengend Snippet: Enterolactone inhibits IGF-1-induced tyrosine phosphorylation of IGF-1R in a dose- (A,B) and time-related (C,D) manner. (A) PC-3 cells were starved in serum-free medium for 24 h and pretreated with indicated doses of enterolactone for 0.5 h followed by stimulation with IGF-1 for 15 min. After treatment, cellular proteins were isolated and immunoprecipitation and immunoblotting were performed. (B) Quantified data of A. The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc densitometry software (Quantity One, version 4.5.0; Bio-Rad). The densitometry data were normalized to IGF-1R levels and those of the control (lane 1). (C) PC-3 cells were starved for 24 h, followed by treatment with enterolactone (60 μmol/L) for 0.5 h, then IGF-1 was added, cells were incubated and harvested at various time points, and levels of phospho-IGF-1R and IGF-1R were determined. (D) Quantified data of C. Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.

Article Snippet: The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc densitometry software (Quantity One, version 4.5.0; Bio-Rad).

Techniques: Phospho-proteomics, Isolation, Immunoprecipitation, Western Blot, Software, Control, Incubation

Enterolactone inhibits phosphorylation of AKT (A,C), its downstream targets (B,D,E,G), and phosphorylation of ERK1/2 (F,H). PC-3 cells were starved in serum-free medium for 24 h. Then, cells were pretreated with indicated doses of enterolactone for 0.5 h. IGF-1 was added to stimulate cells for 15 min (A,B,F) and 6 h (E). After treatment, cellular proteins were isolated and immunoblotting was performed. (C,D,G,H) Quantified data of immunoblotting. The densitometry data of phospho-AKT, phospho-p70S6K1, phospho-GSK-3β, cyclin D1, and p-ERK1/2 obtained in A,B,E, and F were normalized to GAPDH levels and those of the control (lane 1). Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.

Journal:

Article Title: Enterolactone Inhibits Insulin-Like Growth Factor-1 Receptor Signaling in Human Prostatic Carcinoma PC-3 Cells 1 2

doi: 10.3945/jn.108.101832

Figure Lengend Snippet: Enterolactone inhibits phosphorylation of AKT (A,C), its downstream targets (B,D,E,G), and phosphorylation of ERK1/2 (F,H). PC-3 cells were starved in serum-free medium for 24 h. Then, cells were pretreated with indicated doses of enterolactone for 0.5 h. IGF-1 was added to stimulate cells for 15 min (A,B,F) and 6 h (E). After treatment, cellular proteins were isolated and immunoblotting was performed. (C,D,G,H) Quantified data of immunoblotting. The densitometry data of phospho-AKT, phospho-p70S6K1, phospho-GSK-3β, cyclin D1, and p-ERK1/2 obtained in A,B,E, and F were normalized to GAPDH levels and those of the control (lane 1). Values are means ± SEM, n = 4. Means without a common letter differ, P < 0.05. ENL, Enterolactone.

Article Snippet: The intensity of phospho IGF-1R and total IGF-1R protein signals obtained in A was quantified using Chem Doc densitometry software (Quantity One, version 4.5.0; Bio-Rad).

Techniques: Phospho-proteomics, Isolation, Western Blot, Control